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    L-Asparagine/L-Glutamine/ Ammonia Assay Kit

    The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).
    SKU: WZZZ2017

    The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).

    Compatible with MegaQuant™  Wave Spectrophotometer

    Advantages

    • Very rapid reaction due to use of uninhibited glutamate dehydrogenase

    • All enzymes supplied as stabilised suspensions

    • Only kit available

    • Very cost effective

    • All reagents stable for > 2 years after preparation

    • Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

    • Mega-Calc™ software tool for hassle-free raw data processing

    • Standard included

     

    Principle:


    (glutaminase)

    (1) L-Glutamine + H2O → L-glutamate + NH4+



    (microbial glutamate dehydrogenase)

    (2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O



    (asparaginase)

    (3) L-Asparagine + H2O → L-aspartate + NH4+

     

    Kit size: 50 assays of each

    Method: Spectrophotometric at 340 nm

    Reaction time: ~ 20 min

    Detection limit: 0.50 mg/L (L-asparagine) 0.54 mg/L (L-glutamine) 0.06 mg/L (ammonia)

     

    DATA BOOKLET 

    SDS 

    MEGA-CALC 

    Megazyme Knowledge Base

     

    The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials. Includes 100 assays (50 of each).

    Compatible with MegaQuant™  Wave Spectrophotometer

    Advantages

    • Very rapid reaction due to use of uninhibited glutamate dehydrogenase

    • All enzymes supplied as stabilised suspensions

    • Only kit available

    • Very cost effective

    • All reagents stable for > 2 years after preparation

    • Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

    • Mega-Calc™ software tool for hassle-free raw data processing

    • Standard included

     

    Principle:


    (glutaminase)

    (1) L-Glutamine + H2O → L-glutamate + NH4+



    (microbial glutamate dehydrogenase)

    (2) NH4+ + 2-oxoglutarate + NADPH → L-glutamate + NADP+ + H2O



    (asparaginase)

    (3) L-Asparagine + H2O → L-aspartate + NH4+

     

    Kit size: 50 assays of each

    Method: Spectrophotometric at 340 nm

    Reaction time: ~ 20 min

    Detection limit: 0.50 mg/L (L-asparagine) 0.54 mg/L (L-glutamine) 0.06 mg/L (ammonia)

     

    DATA BOOKLET 

    SDS 

    MEGA-CALC 

    Megazyme Knowledge Base