Analyzer format UV-method for the determination of acetic acid
in foodstuffs, beverages and other materials. Includes141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL).
Advantages
• No wasted ACS solution (stable suspension supplied)
• PVP incorporated to prevent tannin inhibition
• Very stable reagent when prepared for auto-analyzer applications (> 3 days at 4°C)
• Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
• Validated by the University of Wine, Suze la Rousse, France
• Very competitive price (cost per mL of reagent)
• All reagents stable for > 2 years after preparation
Principle:
(acetyl-CoA synthetase)
(1) Acetic acid + ATP + CoA → acetyl-CoA + AMP + pyrophosphate
(citrate synthase)
(2) Acetyl-CoA + oxaloacetate + H2O → citrate + CoA
(L-malate dehydrogenase)
(3) L-Malate + NAD+ ↔ oxaloacetate + NADH + H+
Kit size: 141.6 mL of prepared reagent (R1 + R2)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 10 mg/L (recommended assay format)
DATA BOOKLET
SDS
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